The Inhibition of Differentiation Caused by TGFβ in Fetal Myoblasts Is Dependent upon Selective Expression of PKCθ: A Possible Molecular Basis for Myoblast Diversification during Limb Histogenesis
Identifieur interne : 001006 ( Main/Exploration ); précédent : 001005; suivant : 001007The Inhibition of Differentiation Caused by TGFβ in Fetal Myoblasts Is Dependent upon Selective Expression of PKCθ: A Possible Molecular Basis for Myoblast Diversification during Limb Histogenesis
Auteurs : Fabrizio Zappelli [Italie] ; Daniela Willems [Italie] ; Shin-Ichi Osada [Japon] ; Shigeo Ohno [Japon] ; William C. Wetsel [États-Unis] ; Mario Molinaro [Italie] ; Giulio Cossu [Italie] ; Marina Bouché [Italie]Source :
- Developmental Biology [ 0012-1606 ] ; 1996.
English descriptors
- Teeft :
- Academic press, Angelis, Bers, Biol, Bouche, Cdna, Cell biol, Cell proliferation, Copyright, Cos1 cells, Cossu, Different isoforms, Differential expression, Differential response, Differential sensitivity, Differentiation, Embryo, Embryo limbs, Embryonic, Embryonic myoblasts, Expression vectors, Fetal, Fetal muscle, Fetal myoblasts, Growth factor, Growth factors, High levels, Histogenesis, Isoform, Isoforms, Kinase, Limb, Molinaro, Mouse embryos, Muscle histogenesis, Myoblast, Myoblasts, Myogenic, Myogenic cells, Myogenic differentiation, Myosin, Myotubes, Osada, Overexpression, Pkcu, Pkcu expression, Positive control, Possible role, Protein kinase, Satellite cells, Signal transduction, Skeletal muscle, Tgfb, Tgfb sensitivity, Total protein lysates, Transduction, Transfected, Western blot, Western blot analysis, Wetsel.
Abstract
Embryonic and fetal skeletal myoblasts are responsible for the formation of primary and secondary fibers in mammals, but the mechanism which diversifies their fate is unknown.In vitro,embryonic myoblasts are resistant to the differentiation inhibitory effects of transforming growth factor β and phorbol esters. Thus, differential expression of specific molecules involved in the transduction of extracellular signals may contribute to the different phenotypes. We report here that protein kinase C θ, but none of the other known protein kinase C isoforms, is selectively expressed in fetal and postnatal muscle cells (at both the myoblast and myotube stage)in vitroandin vivo.By contrast, embryonic myoblasts and myotubes do not express protein kinase C θin vitroorin vivo.This difference is causally related to a differential response to transforming growth factor β, since overexpression of protein kinase C θ, but not of protein kinase C α or ζ, in embryonic myoblasts makes these cells sensitive to transforming growth factor β. These data demonstrate for the first time that a protein kinase C isoform is a key component of the signal transduction cascade which follows exposure of myoblasts to transforming growth factor β. They also suggest a specific role for protein kinase C θ in determining the fate of different myoblasts during muscle histogenesis.
Url:
DOI: 10.1006/dbio.1996.0292
Affiliations:
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Thus, differential expression of specific molecules involved in the transduction of extracellular signals may contribute to the different phenotypes. We report here that protein kinase C θ, but none of the other known protein kinase C isoforms, is selectively expressed in fetal and postnatal muscle cells (at both the myoblast and myotube stage)in vitroandin vivo.By contrast, embryonic myoblasts and myotubes do not express protein kinase C θin vitroorin vivo.This difference is causally related to a differential response to transforming growth factor β, since overexpression of protein kinase C θ, but not of protein kinase C α or ζ, in embryonic myoblasts makes these cells sensitive to transforming growth factor β. These data demonstrate for the first time that a protein kinase C isoform is a key component of the signal transduction cascade which follows exposure of myoblasts to transforming growth factor β. They also suggest a specific role for protein kinase C θ in determining the fate of different myoblasts during muscle histogenesis.</div></front></TEI><affiliations><list><country><li>Italie</li><li>Japon</li><li>États-Unis</li></country><region><li>Caroline du Nord</li></region></list><tree><country name="Italie"><noRegion><name sortKey="Zappelli, Fabrizio" sort="Zappelli, Fabrizio" uniqKey="Zappelli F" first="Fabrizio" last="Zappelli">Fabrizio Zappelli</name></noRegion><name sortKey="Bouche, Marina" sort="Bouche, Marina" uniqKey="Bouche M" first="Marina" last="Bouché">Marina Bouché</name><name sortKey="Cossu, Giulio" sort="Cossu, Giulio" uniqKey="Cossu G" first="Giulio" last="Cossu">Giulio Cossu</name><name sortKey="Molinaro, Mario" sort="Molinaro, Mario" uniqKey="Molinaro M" first="Mario" last="Molinaro">Mario Molinaro</name><name sortKey="Willems, Daniela" sort="Willems, Daniela" uniqKey="Willems D" first="Daniela" last="Willems">Daniela Willems</name></country><country name="Japon"><noRegion><name sortKey="Osada, Shin Ichi" sort="Osada, Shin Ichi" uniqKey="Osada S" first="Shin-Ichi" last="Osada">Shin-Ichi Osada</name></noRegion><name sortKey="Ohno, Shigeo" sort="Ohno, Shigeo" uniqKey="Ohno S" first="Shigeo" last="Ohno">Shigeo Ohno</name></country><country name="États-Unis"><region name="Caroline du Nord"><name sortKey="Wetsel, William C" sort="Wetsel, William C" uniqKey="Wetsel W" first="William C." last="Wetsel">William C. 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